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. Author manuscript; available in PMC: 2012 Jul 5.
Published in final edited form as: Virology. 2011 May 6;415(2):83–94. doi: 10.1016/j.virol.2011.04.002

Fig. 4. Thermolysin-treated ZEBOV-GP pseudovirions remain sensitive to AXL RNAi treatment.

Fig. 4

A and B) Knockdown of Axl expression in SNB-19 cells by validated AXL siRNA (Invitrogen) at 48 hours following transfection as assessed by immunoblotting of cell lysates for Axl (A) or cell surface expression of Axl (B). Shown in the black histogram are cells treated with AXL RNAi; the grey histogram represents cells treated with an irrelevant RNAi. C) Thermolysin treated full length ZEBOV-GP FIV pseudovirions remain sensitive to AXL RNAi treatment. Virions were treated with thermolysin or mock treated and added to SNB-19 cells that were treated with either AXL RNAi or an irrelevant RNAi. Cells were evaluated at 48 hours following transduction for β-gal expression. The findings are shown as the ratio of the number of transduced cells found in each treatment divided by control values. The numbers in parenthesis represent the effect of AXL RNAi treatment on transduction relative to the respective control. **, p <0.001.