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. Author manuscript; available in PMC: 2012 Jul 5.
Published in final edited form as: Virology. 2011 May 6;415(2):83–94. doi: 10.1016/j.virol.2011.04.002

Fig. 7. Axl transiently associated with ZEBOV-GP ΔO pseudovirions on the cell surface and is internalized as virions enter cells.

Fig. 7

Confocal microscopy of Axl and FIV capsid during ZEBOV-GP ΔO FIV transduction. A) Localization of Axl and FIV capsid expression in SNB-19 cells incubated with ZEBOV-GP ΔO FIV (MOI=250) for 1 hour at 4°C. B–D) Localization of Axl and FIV capsid in SNB-19 cells following incubation of ZEBOV-GP ΔO FIV (MOI=250) for 1 hour at 4°C, removal of unbound virions and incubation of cells with pre-warmed media at 37°C for 15 minutes (B), 30 minutes (C) or 45 minutes (D). All coverslips were fixed with 2% paraformaldehyde, permeabilized with 0.2% Triton X-100 and immunostained with goat anti-Axl and mouse anti-FIV capsid. Findings shown in panels are representative experiments performed three independent times.