FIG. 2.

15-epi-LXA4 stimulates endothelial cell proliferation. First passage of HCE cells derived from a 40-year-old donor were plated in a 12-well plate and grow to 60%–70% confluence. After starving for 24 h, the cells were incubated in Dulbecco's modified Eagle's medium/F12 with different concentrations of 15-epi-LXA4 for 24 h at 37°C. Then, the cells were immunostained with Ki-67 monoclonal antibody as explained in Methods section. Cell proliferation was expressed as the percentage of Ki-67 positive cells versus the total cells. The bars represent the average±SD of 10 fields randomly chosen, *P<0.05 compared with control. The experiment was repeated 4 times.