FIG. 3.

15-epi-LXA4 has no effect on endothelial cell migration. First passage of HCE cells derived from a 63-year-old donor were cultured to complete confluence. After starving for 24 h, a linear wound was made as explained in Methods section. Then, the cells were incubated in Dulbecco's modified Eagle's medium/12 with or without 100 nM 15-epi-LXA4 for 24 h at 37°C. Cells were observed under the microscope and photographed. The migration was expressed as wound width, calculated with an Adobe Photoshop program. Each bar represents the average±SD of 8 pictures of areas randomly chosen. The experiment was repeated thrice with similar results.