FIG. 5.

Effect of E2 on VEGF and PEDF level in Cell Media. RhREC were grown for 24 h in a medium containing 0.0, 0.1, 1.0, or 10.0 nM E2. ELISA was performed and the cell medium was used to quantify VEGF (A) and PEDF (B) in the cell medium. (A) VEGF levels were significantly less in the medium of cells grown in a cell medium containing 1.0 nM E2, (P<0.01,∗∗), or 10.0 nM E2, (P<0.001,∗∗∗), compared with those grown in 0.0 nM E2. (B) PEDF levels were significantly less in the medium of cells grown in a growth medium containing 1.0 nM, (P<0.01,∗∗), or 10.0 nM E2, (P<0.01,∗∗), compared with those grown in 0.0 nM E2. Mean±standard error (n=3) from one experiment is shown. Data are normalized based on the number of viable cells in the assay. Cytokine concentrations/10,000 cells were determined by dividing the VEGF or PEDF concentration in each individual well by the cell count to find cytokine concentration/cell and then multiplying this number by 10,000. Similar experiments were completed 3 times with equivalent results. VEGF, vascular endothelial growth factor; PEDF, pigment epithelium-derived factor.