FIG. 7.

Effect of T and R on E2-induced changes in PEDF levels in cell media. RhREC were grown for 24 h in a medium containing either 0.0 or 1.0 nM E2 alone or 1.0 nM E2 with 0.01, 0.1, 1.0, or 10.0 nM T (A) or R (B). ELISA was performed and the cell medium was used to quantify PEDF. (A) PEDF levels were significantly greater in the 1.0 nM E2 group with 10.0 nM R group compared to the 1.0 nM E2 alone group (P<0.01,∗∗). (B) PEDF levels were significantly increased in the 1.0 nM E2 with 1.0 nM R, (P<0.01,∗∗), and in the 1.0 nM E2 group with 10.0 nM R group, (P<0.01,∗∗), compared to the 1.0 nM E2 alone group. Mean±standard error (n=3) from one experiment is shown. Data are normalized based on the number of viable cells in the assay. Cytokine concentrations/10,000 cells were determined by dividing the PEDF concentration in each individual well by the cell count to find cytokine concentration/cell then multiplying this number by 10,000. Similar experiments were completed 3 times with equivalent results.