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. Author manuscript; available in PMC: 2012 Aug 3.
Published in final edited form as: Physiol Behav. 2011 Jan 12;104(2):327–333. doi: 10.1016/j.physbeh.2011.01.003

Figure 4.

Figure 4

Lack of GABAB Receptor Regulation of Newborn Male CRH Immunoreactivity in the PVN. P0 male mice that lacked functional GABAB receptors (C and D) had the same amount of immunoreactive CRH peptide as wild type littermates (A and B). There was no statistically significant difference between genotypes when the antiserum was used at either the 1:50,000 dilution (A and C) or the 1:100,000 dilution (B and D). Data are from neuron counts in images after thresholding in Metamorph and are displayed graphically in panel E. There were 3 animals in each group. Error bars represent standard error of the mean and scale bars indicate 100 μm. Third ventricle; V.