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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Neurochem. 2011 Mar 7;118(5):826–840. doi: 10.1111/j.1471-4159.2011.07210.x

Figure 2. Aβ25-35-treated microglia potentiate the reduction of astroglial coupling induced by hypoxia in high glucose.

Figure 2

(A–F) Averaged data normalized to control (dashed line) of area of LY diffusion in astrocytes alone (Ast) or astrocytes co-cultured for 24 h with microglia (MG) in presence or absence of 10 μM Aβ25-35 (Aβ) and then exposed to 3 h hypoxia in 5 mM (○) or 27 mM (●) glucose followed by several periods of reoxygenation. It is shown the LY diffusion between astrocyte pre-treated for 24 h with 10 μM Aβ25-35 or with conditioned media from microglia exposed for 24 h to 10 μM Aβ25-35 (CM-Aβ) and then subjected to hypoxia/reoxygenation. It is also shown the LY diffusion between astrocytes pre-treated for 24 h with TNF-α and IL-1β (10 pg/ml of each).** p < 0.005, *** p < 0.001, (●) vs (○) at each time point. Each value corresponds to mean ± S.E. of four independent experiments.