Figure 8. Aβ25-35-treated microglia increase the release glutamate and ATP from astroglial Cx43 hemichannels during reoxygenation.
Release of glutamate (black bars) and ATP (gray bars) by astrocytes under control conditions or treated with CM-Aβ for 24 h and then subjected to 3 h hypoxia in 5 mM glucose followed by 1 h reoxygenation. In some experiments 200 μM Gap26, 500 μM probenecid (Prob), 200 μM 10panx1 and 10 μM brefeldin A (Bref A) were applied during the reoxygenation period. Also is shown data from astrocytes from Cx43−/− mice subjected to the same abovementioned protocols.