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. Author manuscript; available in PMC: 2011 Jun 5.
Published in final edited form as: Methods Mol Biol. 2010;637:121–132. doi: 10.1007/978-1-60761-700-6_6

Fig. 3. Evaluation of ABCB1 gene expression in KB-V1 cells using SYBR Green and TaqMan qRT-PCR.

Fig. 3

The delta-delta CT method was used to determine the fold change for ABCB1 gene expression in KB-V1 cells compared to the parental KB-3-1. The SYBR Green method utilized 300 ng of Total RNA whereas the TaqMan used 40 ng of cDNA. Both were normalized to the equivalent amounts of PMCA4 as the reference gene. The values represent the mean and standard deviation (n=2). (Calcagno AM and Ambudkar SV, unpublished data).