Analysis of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative RT-PCR

. Author manuscript; available in PMC: 2011 Jun 5.

Published in final edited form as: Methods Mol Biol. 2010;637:121–132. doi: 10.1007/978-1-60761-700-6_6

cDNA from Step 3.3.1 (40 ng cDNA)	2 μl
TaqMan Universal PCR Master Mix	25 μl
20X TaqMan Gene Expression Assay Mix	2.5 μl
RNAse-free water	20.5 μl
TOTAL	50 μl