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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Pulm Pharmacol Ther. 2010 Feb 23;23(5):456–464. doi: 10.1016/j.pupt.2010.02.003

Figure 3. MCT induces an increase in ERK protein levels and a decrease in activated (or phosphorylated) Smad1 protein in whole rat lung.

Figure 3

mRNA and protein were collected from whole lung from rats injected with MCT at the indicated timepoints after injection. The week 0 group was injected with saline, and protein and mRNA was collected the next day. mRNA was used in RT-PCR to quantify ERK1 mRNA levels (A) using the primer listed in Table 1. This signal was normalized against GAPDH mRNA levels. Protein lysate resolved electrophoretically was transferred to a nitrocellulose membrane which was immunoblotted for phosphorylated Smad1 (p-Smad1) and ERK. Representative gels are shown in (Ba) and summarized data in (Bb and Bc). Protein signals were normalized to a-tubulin protein levels. Data are presented in arbitrary units (a.u.) as mean ± SD; n=5/group; ** P<0.01 vs. 0 week control group.