Fig. 3.

The deletion of atfA causes derepression of sexual development.

A. CLK43 (WT) and TFLΔatfA-02 (ΔatfA) strains were induced to undergo sexual development in confluent plates as previously reported (Kawasaki et al., 2002). Pictures were taken from confluent cultures after 10 days of induction. Under these conditions the ΔatfA mutant produced more Hülle cells and cleistothecia (black spheres; some in circles) than the WT.

B. Strains CLK43 (WT) or TFLΔatfA-02 (ΔatfA) were induced to undergo sexual development as (A) and samples were taken every 24 h (see Experimental procedures). The total number of cleistothecia per fixed area was counted under a dissection microscope and used to calculate cleistothecia cm⁻² as reported (Kawasaki et al., 2002). Data are mean values from three independent experiments; bars indicate standard deviation.