TNF-α is necessary for phthisis but not rejection of intraocular Ad5E1 clone 2.1 tumors. A, clone 2.1 tumor cells, I/CB cells, and corneal endothelial cells were incubated in medium alone or medium containing TNF-α for 48 hours. Cytotoxicity was determined by LDH release. B, Ad5E1 clone 2.1 tumor cells were injected into the AC of TNF-α KO mice or WT C57BL/6 mice. C, photograph of nonphthisical rejection of Ad5E1 clone 2.1. D, AC injection of clone 2.1 tumor cells in WT, TNF-α KO, TNFR1 KO, and TNFR2 KO mice. Experiments were performed twice with similar results. n = 5 mice per group.