Table 1.
Relative expression levels of MDV1 microRNAs in tumors produced by a very virulent + strain (vv+, 615K) compared to a very virulent strain (vv, RB1B) strain
| microRNA | vv+/vv |
|---|---|
| Upstream of meq | |
| mdv1-miR-M2-5p | 3.9 |
| mdv1-miR-M2-3p | 6.4 |
| mdv1-miR-M3-5p | 1.6 |
| mdv1-miR-M4-5p | 3.3 |
| mdv1-miR-M5-3p | 3.2 |
| mdv1-miR-M12-3p | 2.3 |
| LAT region | |
| mdv1-miR-M6-5p | 0.9 |
| mdv1-miR-M6-3p | 1 |
| mdv1-miR-M7-5p | 1.1 |
| mdv1-miR-M7-3p | 0.7 |
| mdv1-miR-M8-5p | 0.9 |
| mdv1-miR-M8-3p | 0.9 |
Ratios were derived from average densitometry of northern blot analysis of samples from two different chickens for each strain. Expression of viral DNA was comparable in all samples. U6 was used as a loading control and showed a ratio of 0.8.