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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Mol Cell. 2011 May 6;42(3):297–307. doi: 10.1016/j.molcel.2011.03.020

Figure 1.

Figure 1

Telomerase action is processive in H1299 cells. (A) Strategy to study telomerase action on lagging daughter telomeres. Extended lagging overhangs have higher density than unextended overhangs because of the incorporation of BrdU by telomerase. (B) Predicted results for processive and distributive manner of telomerase action when 50% and 75% of activity was inhibited. (C) TRAP assay for telomerase activity in the cells with and without GRN163L treatment. 0.5 and 1.0 µM of GRN163L were used to obtain approximately 50% and 70% of inhibition. (D) CsCl overhang assay of telomere extension in H1299 cells with and without treatment of GRN163L that partially inhibit telomerase activity. See also supplementary Fig. S1.