Table 1.
Tryptophan fluorescence of FtsZ trp mutant (L189W, L160W, Y222W and L68W) quenched by 100 mM acrylamide with or without assembly in GTP. The numbers in columns 2,3,5,6 are the fluorescence emission intensity in arbitrary units, but all scaled together. The quenching % is the percent reduction in acrylamide.
| Mutant | GDP | GDP Acrylamide | Quenching % – GDP | GTP | GTP Acrylamide | Quenching % – GTP |
|---|---|---|---|---|---|---|
| L189W | 131 | 104 | 20.6% | 317 | 275 | 13.2% |
| L160W | 260 | 196 | 24.6% | 347 | 260 | 25.1% |
| Y222W | 302 | 150 | 50.3% | 180 | 126 | 30.0% |
| L68W | 390 | 214 | 45.1% | 1004 | 817 | 18.6% |