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. Author manuscript; available in PMC: 2012 May 25.
Published in final edited form as: J Am Chem Soc. 2011 Apr 29;133(20):7656–7659. doi: 10.1021/ja201031g

Figure 5.

Figure 5

Epi-fluorescence microscopy images of the nanoimprinted nanoarray functionalized with Rhodamine Red-labeled dsDNA, exhibiting the PvuII recognition site (green channel), and Cy3-labelled dsDNA lacking the PvuII recognition site (red channel): 100 ms exposure time; a) and b) show the nanoarray before the adding of the enzyme, while c) and d) show how within seconds of the addition of the enzyme the PvuII active DNA is cleaved, as evidenced by the loss of fluorescence (green channel), while the PvuII inactive DNA is not affected by the presence of the enzyme (red channel).