Figure 4. Acute DFP intoxication injures a subpopulation of neurons.
Representative photomicrographs of (A) Fluoro-Jade B (FJB) labeling and (B) DAPI staining in the hippocampal CA1 region of a rat 24 h post-DFP injection. All animals were anesthetized and pretreated with pyridostigmine (0.1 mg/kg, im) and atropine methyl nitrate (20 mg/kg, im) prior to DFP (9 mg/kg, ip) injection. (C) A merged image illustrates that only a subpopulation of DAPI-positive cells is labeled with FJB. Bar = 100 μm.