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. Author manuscript; available in PMC: 2012 Jun 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Apr 12;253(3):261–269. doi: 10.1016/j.taap.2011.03.026

Figure 8. Non-neuronal cells are not labeled by Fluoro-Jade B (FJB) in brains from rats acutely intoxicated with DFP.

Figure 8

Anesthetized rats were pretreated with pyridostigmine (0.1 mg/kg, im) and atropine methyl nitrate (20 mg/kg, im) 30 or 10 min, respectively, prior to ip injection of DFP (9 mg/kg). Brain sections collected at 24 h post-injection were labeled with FJB and immunostained for the astrocyte-selective antigen GFAP (A), the oligodendrocytes antigen O4 (B), the activated macrophage antigen ED1 (C) or the microglia antigen CD11b (D). FJB labeling and immunoreactivity for these various markers were examined in the amygdala, cortex, thalamus and hippocampus from three DFP-treated rats. Representative images from the hippocampus CA1 (A), cingulate cortex (B), dentate gyrus (C) and somatosensory cortex (D) illustrate the general finding across all four brain regions that FJB-labeled cells do not co-localize with GFAP, O4, ED1 or CD11b-immunoreactivity. Bar = 100 μm.