Table 2.
Distribution of Control Gene Clusters
NR. | Gene ID | Copies | Subset (%) | Library (%) |
---|---|---|---|---|
1 | Ef1_α | 669 | 32.82 | 0.876 |
2 | Cytochrom_cox_I | 274 | 13.55 | 0.362 |
3 | clone_190B1 | 254 | 12.52 | 0.334 |
4 | tubulin_β | 207 | 10.20 | 0.272 |
5 | 40SRibo_protS6 | 183 | 9.02 | 0.241 |
6 | 40SRibo_protS4 | 100 | 4.93 | 0.132 |
7 | 60SRibo_protL4 | 85 | 4.24 | 0.113 |
8 | GAPDH | 82 | 4.04 | 0.108 |
9 | Ef1_β | 67 | 3.30 | 0.088 |
10 | human_calmodulin | 32 | 1.58 | 0.042 |
11 | heat_shock_cogKD71 | 28 | 1.38 | 0.037 |
12 | heat_shock_cogKD90 | 26 | 1.28 | 0.034 |
13 | human_TNF_receptor | 12 | 0.59 | 0.016 |
14 | clone_244D14 | 8 | 0.44 | 0.012 |
15 | clone_241F17 | 2 | 0.10 | 0.003 |
Total | 2029 | 100 | 2.67 |
The entire human cNDA clone set contains 76,032 clones. The subset of the control gene cDNA clones contains 2,029 clones. Most of the control genes are house-keeping genes that have a moderate to high copy rate although three of the genes have a copy rate <15. The copies of the subset sum to a proportion of 2.67% of the entire library.