Table 2.

PCR Primers Prepared for Evaluation of Expressed Sequences and Their Location to the Physical Map

Sequence name	Primer		Ta (C)a	Prod size (bp)	YAC testingbc	Contig testingbc	Source
forward 5′ → 3′	reverse 5′ → 3′
D78212	TCCCAAAACAGAAAGAGCAGATGT	GTGGGGCAGGTGTTCCAATAAA	50	121	932 e 9	−	Chinen et al (1996)
D78215	CCACCGGCAGGAATGAACAAC	CGCTCTAGTGCCGTCTGAGTCTGT	60	165	−	−	Chinen et al (1996)
D78216	ATCAGTACAAGGGGATTCTAACT	ATGTTTTCTTAACGAGGAGACA	50	82	−	−	Chinen et al (1996)
D78221	CCCCTCAGGATTGACTAT	TCACGTCACCTTTTGCAT	50	104	−	−	Chinen et al (1996)
D78225	ACAGCCCCTCAGGATTGACTA	GAGGCATCATCGAAGGTAAGG	55	88	−	−	Chinen et al (1996)
D78226	AGAAGCCCCGAAGAGACACCAA	GGCGCTTTGCCAGGGAAGA	55	134	−	−	Chinen et al (1996)
D78227	GCACCGTTAACAACATGTCTATTT	GAAGAGCCAAGGAAGAGAAACTT	50	143	−	−	Chinen et al (1996)
D78228	TTCAGGTCATTTTTCCAGTTT	CTTCCCTATAGAACGACTTG	50	90	−	−	Chinen et al (1996)
D78229	ATGCGCTACCCCCTGAGAAAAC	GCAGGTGCTGAATCACAACTGTCA	55	147	−	−	Chinen et al (1996)
D78230	TTTTTGAGCTAGAGTTCACATAGA	TGGCAAGCGAATTCACTTCT	50	85	−	−	Chinen et al (1996)
D78231	AGAGGAAAGGCCACATGAGGACA	AGTGTTGGCAGGTCTGATTTCTCC	60	90	−	−	Chinen et al (1996)
D78233	CCTAGGACCCCTGCCTCAG	CCAAAGGGTGGTCAAAGTAGGT	60	81	−	−	Chinen et al (1996)
D78234	TCCTGGCCTAGAGGTCTTA	TGTTTTTGTAGGTCTCAATCTTA	50	103	−	−	Chinen et al (1996)
D78237	TATCTTGAGCCAACCTCCTGTCTC	TCTTGATAGGGGCTGGGTAAAATA	55	103	932 e 9	B-222N15, B-222I10	Chinen et al (1996)
D78239	CTATCCATGCTAAGACATCC	TGTATAAAGGAAAGAAGTTGAATG	50	98	−	−	Chinen et al (1996)
D78242	CGGCCTATGCGACGAGTCA	GTAATTTCGCTGCTTCCAGTCG	50	111	932 e 9	−	Chinen et al (1996)
D78246	TTCTGCTTTTCCTGGACCTGTTCC	CACCGGGCTGGGAGGACTGT	55	123	−	−	Chinen et al (1996)
D78247	CCCCAGTTGAGCCATGAGATAA	TTGGCAATTTCTGTAAGTCAGGAA	55	123	−	−	Chinen et al (1996)
R80652	GTCTGATGTCACTCAGATTGAAGG	TGAGCAAAAAGTCTATGCCTAAA	55	109	932 e 9	B-350A20	Chinen et al (1996)
WI-17604	TGGGCCAAGAGCTTTCTG	TAGACTACTTTGGAGATGCAGCC	50	139	932 e 9	B-350A20	Hum. Gene Map, RHdb
20B08e1	CAATGAGAGGGGCTTTGT	GTTTCCATGGGATGTTCA	50	70	932 e 9	P-20B08	exon amplification
20B08e4	ACTGGCCACCACCAGCGTGAT	GAGCAAGATGGCAGGGGTGGTGTG	55	102	932 e 9	P-20B08	exon amplification
20B08e5	GGGAATTTTAGGTGGTGGTGGTTC	AGGATTCAGGCATAATGGTTTAGC	55	191	932 e 9	P-20B08	exon amplification
R26196	GAATCGTTCCATCATATCCACA	TGACAGCAGGTAACCGATACC	55	201	932 e 9	B-33B22, P-20B08	RHdb

^a(Ta) Annealing T. 

^bThe YAC or BAC names indicate that the EST tested positive with this clone. 

^cNegative signs indicate that the EST did not test positive with any YAC clone or bacterial contig.