Figure 3. The effects of an ROS donor on paw withdrawal response in non-ligated mice.
Response rates to von Frey filament 3.0 were measured prior to and after injection of an ROS donor. A single intrathecal injection of 0.05, 0.10, or 0.25 μg t-BOOH dissolved in 5 μl saline (n = 8, 9, 9, respectively) dose-dependently increased response rates, which lasted up to 1.5 h. Vehicle injection did not affect response rates (n = 9). Data are presented as means ± SEM. Arrow with it, intrathecal injection; *, the value is significantly (p < 0.05) different from that of the vehicle control by two-way repeated-measures ANOVA followed by Duncan's post hoc tests.