Fig. 4.

TUNEL assay of glomerular cell apoptosis. A–E display the representative micrographs of TUNEL-stained glomeruli from vehicle-treated controls (CR), aldosterone-infused rats (ARR), and eplerenone (A/ERR)-, amlodipine (A/ARR)- and telmisartan (A/TRR)-treated animals, respectively. Black arrows indicate apoptotic podocytes in aldosterone-infused rats. F. Comparison of apoptosis ratio of podocyte on a glomerular cross section. Values represent means ± SD. a, P < 0.01 vs. CR; b, P < 0.01 vs. ARR; c, P < 0.01 vs. A/ERR (original magnification × 400).