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H2A variants from CLL patient cells screened on three different days. Histone H2A distributions were screened by LC-MS for 4 healthy volunteers and three groups of CLL samples (12–14 in each group). Four examples from each group are shown for comparison. Patient samples were run on different days to evaluate the reproducibility of the LC-MS method. Two variants of H2A were consistently present at decreased abundance in the CLL patient cells.
