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. Author manuscript; available in PMC: 2011 Oct 15.
Published in final edited form as: Cell. 2011 Apr 15;145(2):257–267. doi: 10.1016/j.cell.2011.03.036

Figure 5. Translocation Velocities at Different Forces and Nucleotide Concentrations.

Figure 5

(A) Histograms with distributions of ClpXP translocation velocities, weighted by the length translocated at that velocity, are shown for experiments using 2 mM ATP in four force regimes. The solid lines are Gaussian fits.

(B) Red symbols are mean velocities from Gaussian fits (± 95% confidence interval), calculated from the panel-A data. The solid line is a non-linear least-squares fit of the force dependence to a single-barrier Boltzmann model (see text). The blue bar represents a range of unloaded velocities from single-molecule and ensemble experiments using different substrates (Martin et al., 2008c; Shin et al., 2009).

(C) Translocation-velocity distributions at 8–12 pN for experiments performed using 75 μM ATP (frequent rupture of bead linkage occurred when less ATP was used) or 1 mM ATP plus 1 mM of ATP S. The dashed vertical line represents the mean velocity for 8–12 pN using 2 mM ATP. Solid lines are Gaussian fits.