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. Author manuscript; available in PMC: 2011 Jun 6.
Published in final edited form as: Basic Clin Pharmacol Toxicol. 2008 Dec;103(6):553–559. doi: 10.1111/j.1742-7843.2008.00316.x

Fig. 3. Genistein induction of hSULT1A1 in Caco-2 cells.

Fig. 3

(A) Enzymatic assay showing dose-dependent induction of hSULT1A1 (P-PST) enzymatic activity in Caco-2 cells treated with different doses of genistein for 7 days. The 2-naphthol sulfation activity of cell cytosol was determined using the radioactive enzyme assay method. (B) Real-time RT-PCR showing the dose-dependent induction of human sulfotransferase hSULT1A1 mRNA expression in Caco-2 cells treated with different doses of genistein for 7 days. (C) Representative Western blot of Caco-2 cell cytosol. (D) Densitometry analysis showing the dose-dependent induction of hSULT1A1 protein expression in Caco-2 cells treated with different doses of genistein for 7 days. Each experiment was repeated at least three times and data presented are means ± S.E.M. of the data collected separately from at least three independent experiments (*P<0.05, **P<0.01, ***P<0.001).

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