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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Immunol. 2011 Apr 27;186(11):6227–6237. doi: 10.4049/jimmunol.1002950

Figure 1. Impaired conjugate efficiency in the absence of ADAP and SKAP55 interaction.

Figure 1

(A) Naïve hCAR/DO11.10 (WT) or hCAR/DO11.10/ADAP−/− (KO) CD4 T cells were transduced with control (Ctrl) Thy1.1 adenovirus or the indicated ADAP or SKAP55 expression construct(s). After 3d, the transduced cells were incubated with OVAp-pulsed splenic B cells and analyzed by flow cytometry for efficiency of T:APC conjugate formation as described in Material and Methods. (B) Intracellular staining for ADAP or SKAP55 was performed on the cells transduced in (A), and plotted versus Thy1.1 expression. Conjugates depicted in A were gated on the top one-third of the Thy1.1 gate to ensure that SKAP55 and/or ADAP levels were comparable to endogenous SKAP55 and/or ADAP expression in wild-type control cells. Results for both the conjugate assays and the intracellular staining are representative of at least 3 independent experiments.