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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Immunol. 2011 Apr 27;186(11):6227–6237. doi: 10.4049/jimmunol.1002950

Figure 6. The SKAP55 PH domain suppresses endogenous NF-κB activation.

Figure 6

(A) Naïve wild-type hCAR T cells were transduced with adenoviruses to overexpress SKAP55 (F-SK55), GFP-SKAP55 (GFP SK55), or GFP-SKAP55-R131M (GFP R131M). Upper 2 panels, Western blots were performed as in A and B to detect total IκBα or phosphorylated IκBα (p-IκB). Lower 2 panels, Western blots for SKAP55 and ADAP are depicted showing expression of endogenous SKAP55 (Endog. SK55), FLAG-SKAP55 (F-SKAP55), or GFP-SKAP55 (GFP-SKAP55) and equal expression of ADAP in all samples. Results are representative of 3 independent assays performed. (B) Quantification of total IκBα degradation following CD3/CD28 stimulation as described in A. Results are normalized to IκBα densitometry values from the unstimulated samples, and averaged between 3 independent experiments performed. Asterisk (*) indicates P < 0.05 compared to the wild-type control condition within each time point using Bonferroni’s multiple comparison test of the one-way ANOVA. n.s., P>0.05.