Table 1.
Oligonucleotide Primers Used to Amplify Mitochondrial Genomic Equivalents from Chimpanzee Fecal DNA.
| Forward Primerb | Reverse Primerb | Sizec | |
| Fragment (first round PCR)a | |||
| A | Ptrg2110U: 5′-TGAAGAGGCGGGCATAACATA-3′ | Ptrg8422L; 5′-GACGTACGGCTAAGGCTATTGG-3′ | 6,311 |
| C | Ptrg11253U: 5′-GACTCCTGGCAAGCCTCGCTAA-3′ | Ptrg2254L; 5′-GTTGTGCTCCGAGGTCGC-3′ | 7,556 |
| D | Ptrg1857U; 5′-ATGCCCACAAGGAAAGGT-3′ | Ptrg7355L; 5′-AAGATTAGCCCGCCGTAG-3′ | 5,499 |
| E | Ptrg4072U; 5′-CACAAGCAACTGCGTCCAT-3′ | Ptrg9714L; 5′-GTAGGGCTCAAGGTAAGG-3′ | 5,643 |
| F | Ptrg9335U; 5′-CGCCGCCTGATACTGRCA-3′ | Ptrg14120L; 5′-TGGTCGTGGTTGTAGTCC-3′ | 4,786 |
| Fragment (second round PCR)a | |||
| A2 | Ptrg2440U; 5′-CGCTATTAAAGGTTCGTTTG-3′ | Ptrg3890L; 5′-ATTAGTACGGGAAGGGTRTAAC-3′ | 1,429 |
| A3 | Ptrg3791U; 5′-TCCGTGCCACCTATCACA-3′ | Ptrg5161L; 5′-TGCGGCGGGAGAAGTAGATTGA-3′ | 1,371 |
| A5 | Ptrg4664U; 5′-CTTCTTACCCAAATGAGTTATC-3′ | Ptrg5556L; 5′-CCAAAGCCTCCGATTAT-3′ | 893 |
| A6 | Ptrg5323U; 5′-GTTCACCGACCGCTGACT-3′ | Ptrg6495L; 5′-CCTCCTATGATGGCGAATACAG-3′ | 1,473 |
| A7 | Ptrg6229U; 5′-CTATTTCACCTCCGCTACCA-3′ | Ptrg8308L; 5′-TAGTCTTAAAGCGAAAGCCTAT-3′ | 2,080 |
| A9 | Ptrg7233U; 5′-CCTACCATCCCTGCGTAT-3′ | Ptrg8422L; 5′-GACGTACGGCTAAGGCTATTGG-3′ | 1,190 |
| C1 | Ptrg11253U; 5′-GACTCCTGGCAAGCCTCGCTAA-3′ | Ptrg13001; 5′-GTGGCGATGAGAGTAATAGATA-3′ | 1,749 |
| C3 | Ptrg12146U: 5′-TAGTCACCGCTAACAACCTA-3′ | Ptrg14105L; 5′-TCCGTGCGAGAATAATGACATA-3′ | 1,960 |
| C4 | Ptrg13976U; 5′-CCAACTACACCACTAACAA-3′ | Ptrg16123L; 5′-TCAAAGACAGATACTGCGACAT-3′ | 2,148 |
| C6 | Ptrg15851U; 5′-AGTGACTCTCCTCGCTCC-3′ | Ptrg358L; 5′-CGCCGGTTTCTATTGAC-3′ | 1,062 |
| C9 | Ptrg126U; 5′-CATCCCCGCCCCGTGAGT-3′ | Ptrg886L; 5′-AGGGCCCTGTTCAACTAAGCAC-3′ | 761 |
| C10 | Ptrg547U; 5′-TGCTCGCCAGAACACTAC-3′ | Ptrg1903L; 5′-GATTTGCCGAGTTCCTTT-3′ | 1,357 |
| D2 | Ptrg1857U; 5′-ATGCCCACAAGGAAAGGT-3′ | Ptrg2884L; 5′-TTATGGCGTCAGCGAARG-3′ | 1,028 |
| E1 | Ptrg8273U; 5′-CAGGCGCAGTAGTCATAG-3′ | Ptrg9524L; 5′-TAGTAGGGCTAGAAGGGTATTG-3′ | 1,252 |
| F1 | Ptrg9335U; 5′-CGCCGCCTGATACTGRCA-3′ | Ptrg10483L; 5′-GAGGGAAATTAGCATGGAGA-3′ | 1,149 |
| F2 | Ptrg10244U; 5′-GCCACCTATCCAACGAA-3′ | Ptrg11468L; 5′-TGAATGAGGGCTTTATGCTATT-3′ | 1,047 |
Note.—a See figure X for fragment position within the P. troglodytes mitochondrial (mt) genome.
b
Primers were designed according to previously published chimpanzee mtDNA sequences (GenBank accession number: D38113) with minor modifications.
c
Size of PCR fragments (in base pairs).