Figure 5.

Effects of hyperforin on apoptosis and the expression of COX-2 and mPGES-1. A549 cells, 60% confluent, were incubated with 2 ng ml⁻¹ interleukin-1β together with vehicle (DMSO) or Hyp in cell culture medium containing 2% (v v⁻¹) fetal calf serum. Cells were harvested after 24 h, total cell lysates were prepared and analyzed for the induction of COX-2 (72 kDa), mPGES-1 (16 kDa) and cleaved PARP (89 kDa) using SDS-PAGE and Western blotting. β-Actin (45 kDa) was used as loading control. Data are representatives of two to three independent experiments.