Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 3;6(6):e20639. doi: 10.1371/journal.pone.0020639

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

(A) Thymocytes from B6, CD28/B7/B6, CD3εKO, and CD28/B7/CD3εKO mice were analyzed for CD4-CD8 subsets (CD4-PE and CD8-Al594) or for lineage negative DN subsets (CD25-PE and CD44-APC). Thymus numbers are to the right of these figures. Staining profiles are representative of >40 mice of each genotype analyzed. (B) Thymi were harvested from mice injected with BrdU, lineage negative cells were gated on the indicated DN subsets, and analyzed for BrdU incorporation. These histograms show the percentage BrdU⁺ cells present in all DN3 cells, early DN3 (CD25^hi) cells, or late DN3 (CD25^low) cells from B6 or CD28/B7/CD3εKO mice. This experiment is representative of three independent experiments performed.