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. 2011 Jun 3;6(6):e20639. doi: 10.1371/journal.pone.0020639

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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100, 30, and 10 ng of DNA was analyzed by PCR to detect (A) Dβ2-Jβ2 rearrangement, (B) Vβ5-DJβ2 rearrangement, (C) Vβ8.1-DJβ2 rearrangement, or (D) Vβb14.1-DJβ2 rearrangement. (E) 10, 3, and 1 ng of DNA was analyzed by PCR to detect unrearranged IgM as a loading control. The arrow in panel A denotes the amplification of germline or non-rearranged Dβ2-Jβ2 PCR products. These results presented here comparing VDJβ rearrangements in CD3ε and CD28/B7/CD3εKO DN cells were analyzed in the same experiment and are representative of three experiments analyzing DNA prepared from a total of six CD3εKO and CD28/B7/CD3εKO mice.