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. 2011 Jun 3;6(6):e20639. doi: 10.1371/journal.pone.0020639

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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300, 100, and 30 ng of DNA prepared from FCM sorted DP cells was analyzed by PCR to detect the indicated Vα-Jα rearrangements. The PCR reaction for unrearranged IgM was performed using 10 ng DNA. The results presented here comparing TCRα rearrangements in B6 and CD28/B7/CD3εKO DP cells were analyzed in the same experiment and are representative of three experiments performed using DNA prepared from five to six B6 or CD28/B7/CD3εKO mice.