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A typical ELISA measuring anti-MT IgG of autistic children with GI disease (A) compared to control (C) children with no GI disease. Positive control is anti-MT IgG Mab and purified MT. Negative control is PBS replacing anti-MT IgG.
Abbreviations: anti-MT, anti-metallothionein; ELISA, enzyme-linked immunosorbent assay; GI, gastrointestinal; IgG, immunoglobulin G; MT, metallothionein; PBS, phosphate buffer solution.
