Figure 2.

Illustration of IBMS CGI gene database. Following the determination of CGI genes, more comprehensive analysis was performed to obtain the possible full-length nucleotide sequences by dbEST searches; to determine the optimum open-reading frame; to search the UniGene-human database for chromosome localization, tissue distribution, and EST matches; and a final BLAST analysis to confirm its novelty and protein family annotation. All information was then stored in a customized FileMaker Pro-based IBMS CGI gene database. Only the basic layout is shown here. There are different easy-viewing layouts designed to store nucleotide and translated protein information, the original C. elegans query protein information (possible ortholog gene), the full-length contig assembly information and the final BLASTP search results.