Fig. 2.

The linoleic acid effect is sustained and reversible. a Rapid and stable current block by 100 μM LA. Oocytes were exposed to 100 μM LA for 5, 15, or 30 min, and hemichannel currents were measured. Data were normalized and expressed as described in the legend to Fig. 1c. The solid lines are the fits of the Boltzmann equation to the data (n=3 each). b Reversibility of the inhibition by LA. Oocytes expressing Cx46 hemichannels (n=12) were exposed to LA for 5 min (100 μM LA), and hemichannel currents were recorded. Currents were measured again after washing without LA for 5 min (Wash). Currents were normalized to control values. A P<0.001 compared to control is denoted by **