Fig. 5.

Microinjection of linoleic acid-CoA increases Cx46 hemichannel currents. a Current–voltage relationship of Cx46 hemichannel currents under control conditions and after a 5-min exposure to 100 μM linoleic acid attached to coenzyme A (LA-CoA) or LA alone added to the extracellular solution (n=8 for each group). b Current–voltage relationship of hemichannel currents under control conditions and 5 min after microinjection of LA-CoA (n=15) or LA (n=14) to reach final concentrations of ca. 250 μM. Intracellular concentrations were estimated assuming a 1-μl oocyte volume. Data are presented as means ± SEM of currents normalized to the control value at +60 mV