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. 2011 Mar 7;107(9):1557–1566. doi: 10.1093/aob/mcr045

Fig. 1.

Fig. 1.

Gene structure of the Papaver somniferum AG gene. (A) Schematic showing how alternative splicing at the 3′ end of the coding sequence generates two transcripts, PapsAG-1 and PapsAG-2 (not drawn to scale). Regions underlined in red are gene-specific fragments used for gene-silencing with VIGS and for in situ mRNA hybridization probes. The region overlined with a red bar indicates the presence of a forward primer outside of the region used in vigs lines for expression analysis. Black arrows indicate the splicing donor site. AG MI, AG Motif I; AG MII, AG Motif II. (B) Alignment of the C-terminal regions of translated PapsAG-1 and PapsAG-2 sequences with AG orthologues from tomato (TAG; Q40168), Antirrhinum majus (AmFAR; CAB42988), Arabidopsis thaliana (AtAG; P17839) and Eschscholzia californica (EScaAG1 and EScaAG2; AAZ53205 and AAZ53206). PapsAG-2 has a 24-amino-acid extension. The conserved AG motifs are indicated.