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. 2011 Mar 7;107(9):1557–1566. doi: 10.1093/aob/mcr045

Fig. 4.

Fig. 4.

Distinct effects of PapsAG-1 and PapsAG-2 gene silencing in capsule development: (A) wild-type stigma; (B) wild-type capsule cut open in transverse orientation showing ovules and septae; (C, E) vigsAG1 deformed stigmas; (D) vigsAG1 capsule cut in longitudinal orientation showing defective ovule development particularly at the distal end; (F) vigsAG1 open flower showing extended gynophores and modified anthers; (G, H) vigsAG2 stigma and open capsule cut in transverse orientation showing the deformed stigma and corresponding ovule defects within the capsule; (I, J) scanning electron micrographs of vigsAG2 stigmas, showing a retracted stigmatic ray (blue arrow) and a stigmatic pore in reverse orientation, i.e. it is directed towards the centre of the stigma (red arrow, I); disorganized stigma with ectopic papillae (blue arrow) and ovule (red arrow, J). Scale bars: (A–E, G, H) = 2 mm; (F) = 5 mm; (I) = 200 µm; (J) = 100 µm.