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. 2011 Jun 3;6(6):e20412. doi: 10.1371/journal.pone.0020412

Figure 2. Tacrolimus reduces tumor vascularity.

Figure 2

MMTVneu tumors from 4 control 20% intralipid mice and 4 tacrolimus treated mice were resected and embedded in paraffin. Immunohistochemistry with antibody to factor VIII was performed as described in “Material and Methods”. Microvessel density was determined by counting the number of microvessels in 3 high-power fields at 200×. A, D) The mean number of microvessels per high power field in control tumors was 104±13. B, D) The mean number of microvessels per high power field in tacrolimus treated tumors was 20±1 (p<0.001). This shows that tacrolimus reduces angiogenesis in vivo. C) Negative control human breast tumor without primary antibody shows no staining.