Fig. 1.
Analysis of biotinylated CSF from controls and MS patients. A, Silver stained 12% gel showing the profile of biotinylated CSF proteins (3 μg) from different samples. B, western/far Western blot of the biotinylated CSF proteins (1 μg) probed with streptavidin-HRP to show the extent of biotinylation. Note the extent of similarity in pattern with silver stained gel on the left. C, western/far Western blot of biotinylated CSF proteins (1 μg) probed with anti-human Ig GAM showing Fc chains of immunoglobulins.