Fig. 2.

Representative two-dimensional blot showing the reactivity of Control and MS-CSF to different brain preparations. Two-dimensional gels of brain homogenate, myelin and myelin-axolemmal complex preparations were subjected to western/far Western blotting, probed first with the biotinylated spondylosis CSF control and developed using streptavidin-HRP (A). The same blots were washed thoroughly and probed again with the paired MS-CSF sample 5282 (B) as described in the methods. (C) Corresponding Coomassie stained two-dimensional gels of brain homogenate, myelin and myelin-axolemmal complexes. Spots that showed reactivity to MS-CSF were numbered. Spots common to both blots were no further assessed. Note the reproducibility of the different spots both on the blots (B) and Coomassie stained gels (C).