Fig. 5.

FVIII-derived MHC class II ligands identified from donor. A The first column shows residue numbers and corresponding domain of the FVIII molecule. The following columns show amino acid sequence, charge, XCorr value as provided by Sequest, retention time (RT), mass-to-charge ratio (m/z) and mass tolerance (ΔM). The two last columns show predicted binding scores to the MHC class II alleles of the donor. Binding scores to corresponding MHC class II alleles of the donor were calculated using Propred and NetMHCIIpan epitope prediction programs. Binding thresholds were defined as ≤500 nm for NetMHCIIpan and as top 3% natural binders for Propred (≥0.023 for DRB1*0101 and ≥0.295 for DRB1*1301). The binding motif of peptides that are predicted to bind above the binding threshold are underlined.