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. 2011 Mar 29;10(6):M110.004549. doi: 10.1074/mcp.M110.004549

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — The methods used for the preparation of membrane proteins. A, shows the sucrose density gradient method, B, shows the Triton X-100 solubilization method and panel C exhibits the carbonate extraction procedure. The procedure used in panels A and B generally used to compare the efficiency of these methods to prepare as many membrane proteins as possible from P. syringae Lz4W. The procedure used in panel C was used for identification of more proteins and validate the protein/peptide identifications.