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. 2011 Mar 21;10(6):M110.005678. doi: 10.1074/mcp.M110.005678

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — A, Footprinting by carboxylic-acid, side-chain modification. Carbodiimide (EDC) modifies acid side chain of proteins in physiological relevant buffer. In the presence of nucleophiles (glycine ethyl ester), reaction occur to give mass shifts 85.0522 Da and 57.0209 Da (hydrolysis product of ester). B, Work flow of MS based carboxyl group protein footprint (1) Modification (2) ESI-MS analysis of modified protein (3) In-gel separation and proteolytic digestion (4) LC-MS analysis of peptides (5) LC-MS/MS analysis of peptides (6) calculation of modification extent.