Fig. 3.

A, Carboxyl group modification extents for Her4 kinase-domain protein. Her4 residue numbering is based on the mature, full length protein minus its signal peptide and matches the numbering used in Qiu et al. (11). The residues involved in dimer interface in crystal structure are labeled in red. The residues from activation loop are labeled in blue. Data represent mean and standard deviation of three independent samples. B, Her4 kinase-domain asymmetry dimer. Based on carboxyl group footprinting results, two residues E690 and E912 are labeled in red. (PDB ID: 3BCE).