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. 2010 Sep 22;30(38):12733–12744. doi: 10.1523/JNEUROSCI.0896-10.2010

Figure 1.

Figure 1.

Altered NLG1 surface membrane levels upon application of chemical treatment designed to induce LTP or LTD, respectively. A, B, Chemical treatment designed to induce LTP significantly increases NLG1/3 at neuronal surface membranes. A, Surface biotinylation analysis of acute hippocampal slices detected against NLG1 and pan-cadherin (loading control). B, Neuronal surface biotinylation analysis of NLG1/3 and pan-cadherin (loading control) in cultured hippocampal neurons. C, D, Chemical treatment designed to induce LTD significantly reduces NLG1/3 from neuronal surface membranes. C, Surface biotinylation analysis of acute hippocampal slices detected against NLG1 and pan-cadherin (loading control). D, Neuronal surface biotinylation analysis of NLG1/3 and pan-cadherin (loading control) in cultured hippocampal neurons. Values reflect relative Western blot signal intensities of surface membrane NLG1/3 protein levels, analyzed using the ImageJ software. Error bars represent the SEM. *p < 0.05; **p < 0.01. E, F, Chemical LTP or chemical LTD induction in acute slices of P30 C57BL/6N mice causes a long-lasting increase or depression of fEPSPs, respectively. E, Chemical LTP induction by treatment with forskolin and rolipram led to a long-term increase of fEPSPs by 30% (average of the normalized fEPSP slopes in the last 5 min of recording: 1.29 ± 0.05, n = 5). F, Chemical LTD induction by DHPG treatment caused long-term depression of fEPSPs by 32% (average of the normalized fEPSP slopes in the last 5 min of recording: 0.76 ± 0.03, n = 4). G, Examples showing recordings of spontaneous mEPSCs from a control cultured hippocampal neuron (upper panel) and a cultured neuron treated with forskolin/rolipram (lower panel). H, Mean ± SEM of interevent intervals of control (DMSO treatment) and forskolin/rolipram-treated neurons; the graph shows a very significant difference between the two means (control: 1041.12 ± 216.29 ms, n = 9; forskolin/rolipram: 321.22 ± 65.63 ms, n = 11; p < 0.01). Forskolin/rolipram treatment induces a drastic increase in synaptic activity. The mean values of mEPSC amplitudes of the two groups of neurons were slightly different; however, this difference was not statistically significant (control: 14.4 ± 1.2 pA; forskolin/rolipram: 17.0 ± 2.0 pA; p = 0.296). Error bars represent the SEM. **p < 0.01.