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. Author manuscript; available in PMC: 2011 Jun 6.
Published in final edited form as: J Immunol. 2010 Oct 1;185(9):5198–5204. doi: 10.4049/jimmunol.1001224

FIGURE 5.

FIGURE 5

L-Arg deprivation inhibits HuR expression by impairing global translation. A, Detection of HuR mRNA levels using real-time PCR after culture of cells in medium with or without L-Arg for 24 and 48 h. B, Pulse-chase analysis for HuR in activated T cells cultured in the presence or absence of L-Arg for 48 h. C and D, HuR mRNA and total mRNA levels were tested in polysomes isolated using sucrose gradients from activated T cells cultured in the presence or absence of L-Arg for 48 h. The experiment was repeated a minimum of three times, with similar results having been obtained.