Table 1. Primer sequences for PDE6β exons.

Exon	Forward primer	Reverse primer	Annealing temperature (°C)
1a	CTGGTTTTCCTGGAAGGT	CTGGCGGTACATGAAGAG	55
1a	AGGATATGCAGGAGAGCAT	CTCCTCAGCACAGAACTAGC	55
2	TCTGCTGGACTGAGCACT	GCAGGTAAAGAGGTGGATG	55
3	GTGCACCTGAGCTTGTGTGT	ACCTACCCAGGTGAGCACAA	60
4	CCACAAGCTCAGATGAAACCT	ATCAGCACAGACCACACGTC	59
5	AAGGAGAAGGTGAGGCTTCC	CTGGTGGAGACCACAGACAG	59
6	GGAACACAGACTGGGAAGAC	AGTGAGTCGGCTTCTGTCTC	57
7–8	ACACACACGTGCAGCCTA	AGTGGCAAAAACGAATTCAC	57
9	AAACTCCAAATGCAGAGAGG	TGCTTCTGTGTGGGGTCT	57
10	AGACCCCACACAGAAGCACT	CTGTGACCCCTCAATGGAC	59
11	ACGGTCATTTGTCTCCAGAT	AGTCAGGCCCACTAAACATC	57
12	AACTGGGCAAGTTCTTCACT	TACTTCCCGTGTGCATTTTA	56
13	GAAGTCCAGGAGACGGTGT	AGGGGTTGGGATGACCTA	57
14	TACCAAGGGCAGCACTCA	CGCCACCATACACAGCTT	58
15	CAGGAGGTCAAGGCTGTATT	CACTGAGTGTCCAGGTCCTT	56
16	CCAAGGACCTGGACACTCA	GTGGGAGCAAGTGTGGAGA	59
17	CCTGGCCCTGTACTTCAA	CAAGGGCTACAGACCAATG	57
18	GAGGCTGAGGCACAAGAATC	ACTGCAGTACCCCCATCCTT	55
19	GGCAACGGACCATTGTTT	TGAGATAAGGACCCCACGAC	59
20	TCCATGAGCACATCTGAGTGA	TCCGGAAACTGATGTTCCTC	60
21	CGAGGTTTCTCCCTTCACAG	TGGCTCTGCTTTTCTCCATT	59
22	TGAGCATAATCAGGGCACAG	TTGGGCTTCCTAACCTCTTG	59

The amplification conditions are described in the Methods.